› 1ua4

X-ray diffraction
1.9Å resolution

Crystal Structure of an ADP-dependent Glucokinase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1ua4/pdb
PDB 1ua4 coloured by chain and viewed from the front.
PDB 1ua4 coloured by chain and viewed from the side.
PDB 1ua4 coloured by chain and viewed from the top.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: implications for a sugar-induced conformational change in ADP-dependent kinase.
Ito S, Fushinobu S, Jeong JJ, Yoshioka I, Koga S, Shoun H, Wakagi T
J. Mol. Biol. 331 871-83 (2003)
PMID: 12909015

Function and Biology Details

Reaction catalysed: 
ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-dependent glucose/glucosamine kinase Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 51.33 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V2Z6 (Residues: 1-455; Coverage: 100%)
Gene names: PF0312, glkA
Sequence domains: ADP-specific Phosphofructokinase/Glucokinase conserved region
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand BGC 1 x BGC
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I222
Unit cell:
a: 112.677Å b: 88.948Å c: 103.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.205
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biochemistry and regulatory functions of bacterial glucose kinases.
Romero-Rodríguez et al. (2015)
2 more

5 mentions without citation

Biomedical and Catalytic Opportunities of Virus-Like Particles in Nanotechnology.
Schwarz et al. (2017)
4 more