› 1u8t

X-ray diffraction
1.5Å resolution

Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide

Released:
DOI: 10.2210/pdb1u8t/pdb
PDB entry 1u8t coloured by chain, front view.
PDB entry 1u8t coloured by chain, side view.
PDB entry 1u8t coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the constitutively active double mutant CheYD13K Y106W alone and in complex with a FliM peptide.
Dyer CM, Quillin ML, Campos A, Lu J, McEvoy MM, Hausrath AC, Westbrook EM, Matsumura P, Matthews BW, Dahlquist FW
J Mol Biol 342 1325-35 (2004)
PMID: 15351654

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142407 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 14.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Flagellar motor switch protein FliM Chains: E, F
Molecule details ›
Chains: E, F
Length: 16 amino acids
Theoretical weight: 1.69 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P06974 (Residues: 1-16; Coverage: 5%)
Gene names: JW1929, b1945, cheC2, fla AII, fla QII, fliM

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 54.28Å b: 53.48Å c: 54.1Å
α: 60.36° β: 60.75° γ: 60.57°
R-values:
R R work R free
0.2 0.196 0.273
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

3 review citations

Three (and more) component regulatory systems - auxiliary regulators of bacterial histidine kinases.
Buelow et al. (2010)
2 more

9 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
8 more