1u2g

X-ray diffraction
2.2Å resolution

transhydrogenase (dI.ADPr)2(dIII.NADPH)1 asymmetric complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD(P) transhydrogenase subunit alpha part 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 384 amino acids
Theoretical weight: 40.32 KDa
Source organism: Rhodospirillum rubrum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2RSB2 (Residues: 1-384; Coverage: 100%)
Gene names: Rru_A2183, nntA1, pntAA
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain
NAD(P) transhydrogenase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 203 amino acids
Theoretical weight: 21.49 KDa
Source organism: Rhodospirillum rubrum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2RSB4 (Residues: 262-464; Coverage: 44%)
Gene names: Rru_A2181, nntB, pntB
Sequence domains: NAD(P) transhydrogenase beta subunit
Structure domains: TPP-binding domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 72.356Å b: 73.766Å c: 205.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.254
Expression system: Escherichia coli