1u1j

X-ray diffraction
2.4Å resolution

A. thaliana cobalamine independent methionine synthase

Released:

Function and Biology Details

Reaction catalysed:
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 765 amino acids
Theoretical weight: 85.37 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50008 (Residues: 1-765; Coverage: 100%)
Gene names: At5g17920, CIMS, MPI7.9, MS1
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P65
Unit cell:
a: 123.465Å b: 123.465Å c: 132.721Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.275
Expression system: Escherichia coli BL21(DE3)