X-ray diffraction
2.55Å resolution

A. thaliana cobalamine independent methionine synthase


Function and Biology Details

Reaction catalysed:
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 765 amino acids
Theoretical weight: 85.37 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O50008 (Residues: 1-765; Coverage: 100%)
Gene names: At5g17920, CIMS, MPI7.9, MS1
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P65
Unit cell:
a: 121.725Å b: 121.725Å c: 132.707Å
α: 90° β: 90° γ: 120°
R R work R free
0.216 0.214 0.26
Expression system: Escherichia coli BL21(DE3)