1txq

X-ray diffraction
1.8Å resolution

Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynactin subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14203 (Residues: 15-107; Coverage: 7%)
Gene name: DCTN1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain
Microtubule-associated protein RP/EB family member 1 Chain: B
Molecule details ›
Chain: B
Length: 86 amino acids
Theoretical weight: 9.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15691 (Residues: 183-268; Coverage: 32%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 53.085Å b: 80.21Å c: 39.239Å
α: 90° β: 108.52° γ: 90°
R-values:
R R work R free
0.203 0.203 0.243
Expression system: Escherichia coli BL21(DE3)