PDBe 1tx4

X-ray diffraction
1.65Å resolution

RHO/RHOGAP/GDP(DOT)ALF4 COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho GTPase-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q07960 (Residues: 234-431; Coverage: 45%)
Gene names: ARHGAP1, CDC42GAP, RHOGAP1
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein
Transforming protein RhoA Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61586 (Residues: 3-179; Coverage: 92%)
Gene names: ARH12, ARHA, RHO12, RHOA
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 66.5Å b: 72Å c: 91.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.215
Expression system: Escherichia coli BL21