X-ray diffraction
2.8Å resolution

HincII bound to Ca2+ and cognate DNA GTCGAC

Source organism: Haemophilus influenzae
Primary publication:
Ca2+ binding in the active site of HincII: implications for the catalytic mechanism.
Biochemistry 43 13256-70 (2004)
PMID: 15491133

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme HincII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 29.82 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*G)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Haemophilus influenzae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I212121
Unit cell:
a: 66.945Å b: 177.18Å c: 254.89Å
α: 90° β: 90° γ: 90°
R R work R free
0.18 0.18 0.286
Expression systems:
  • Escherichia coli
  • Not provided