› 1tuv

X-ray diffraction
1.7Å resolution

Crystal structure of YgiN in complex with menadione

Released:
DOI: 10.2210/pdb1tuv/pdb
PDB 1tuv coloured by chain and viewed from the front.
PDB 1tuv coloured by chain and viewed from the side.
PDB 1tuv coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural and biochemical evidence for an enzymatic quinone redox cycle in Escherichia coli: identification of a novel quinol monooxygenase.
Adams MA, Jia Z
J. Biol. Chem. 280 8358-63 (2005)
PMID: 15613473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable quinol monooxygenase YgiN Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADU2 (Residues: 1-104; Coverage: 100%)
Gene names: JW2997, b3029, ygiN
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand VK3 1 x VK3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P4132
Unit cell:
a: 102.562Å b: 102.562Å c: 102.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Cofactor-independent oxidases and oxygenases.
Fetzner et al. (2010)
3 more

4 mentions without citation

Two novel classes of enzymes are required for the biosynthesis of aurofusarin in Fusarium graminearum.
Frandsen et al. (2011)
3 more