Solution NMR

Solution structure of sigma A region 4 from Thermotoga maritima

Source organism: Thermotoga maritima
Primary publication:
T4 AsiA blocks DNA recognition by remodeling sigma70 region 4.
EMBO J. 23 2952-62 (2004)
PMID: 15257291

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
RNA polymerase sigma factor SigA Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 10.38 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
  • Canonical: P77994 (Residues: 313-399; Coverage: 22%)
Gene names: TM_1451, rpoD, sigA
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing in torsion angle space
Expression system: Escherichia coli