› 1ts0

X-ray diffraction
1.6Å resolution

Structure of the pB1 intermediate from time-resolved Laue crystallography

Released:
DOI: 10.2210/pdb1ts0/pdb
PDB 1ts0 coloured by chain and viewed from the front.
PDB 1ts0 coloured by chain and viewed from the side.
PDB 1ts0 coloured by chain and viewed from the top.
Source organism: Halorhodospira halophila
Primary publication:
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds.
Ihee H, Rajagopal S, Srajer V, Pahl R, Anderson S, Schmidt M, Schotte F, Anfinrud PA, Wulff M, Moffat K
Proc Natl Acad Sci U S A 102 7145-50 (2005)
PMID: 15870207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P63
Unit cell:
a: 66.833Å b: 66.833Å c: 40.947Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.117 0.109 0.116
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Advances in methods for atomic resolution macromolecular structure determination.
Thompson et al. (2020)
19 more

6 mentions without citation

Proton transfer reactions and hydrogen-bond networks in protein environments.
Ishikita et al. (2014)
5 more