X-ray diffraction
2.1Å resolution

Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.7 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
  • Canonical: P00644 (Residues: 83-226; Coverage: 67%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 31.298Å b: 60.981Å c: 38.789Å
α: 90° β: 96.1° γ: 90°
R R work R free
0.224 0.212 0.25
Expression system: Escherichia coli BL21