› 1tn0

X-ray diffraction
2.5Å resolution

Structure of bacterorhodopsin mutant A51P

Released:
DOI: 10.2210/pdb1tn0/pdb
PDB 1tn0 coloured by chain and viewed from the front.
PDB 1tn0 coloured by chain and viewed from the side.
PDB 1tn0 coloured by chain and viewed from the top.
Source organism: Halobacterium salinarum
Primary publication:
Proline substitutions are not easily accommodated in a membrane protein.
Yohannan S, Yang D, Faham S, Boulting G, Whitelegge J, Bowie JU
J Mol Biol 341 1-6 (2004)
PMID: 15312757

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.96 KDa
Source organism: Halobacterium salinarum
Expression system: Escherichia coli
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 2 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 45.105Å b: 108.582Å c: 55.821Å
α: 90° β: 113.74° γ: 90°
R-values:
R R work R free
0.177 0.173 0.262
Expression system: Escherichia coli

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Citations

5 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
4 more