› 1tmx

X-ray diffraction
1.75Å resolution

Crystal structure of hydroxyquinol 1,2-dioxygenase from Nocardioides Simplex 3E

Released:
DOI: 10.2210/pdb1tmx/pdb
PDB entry 1tmx coloured by chain, front view.
PDB entry 1tmx coloured by chain, side view.
PDB entry 1tmx coloured by chain, top view.
Source organism: Pimelobacter simplex
Primary publication:
Crystal structure of the hydroxyquinol 1,2-dioxygenase from Nocardioides simplex 3E, a key enzyme involved in polychlorinated aromatics biodegradation.
Ferraroni M, Seifert J, Travkin VM, Thiel M, Kaschabek S, Scozzafava A, Golovleva L, Schlömann M, Briganti F
J Biol Chem 280 21144-54 (2005)
PMID: 15772073

Function and Biology Details

Reaction catalysed: 
Hydroxyquinol + O(2) = maleylacetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyquinol 1,2-dioxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 32 KDa
Source organism: Pimelobacter simplex
UniProt:
  • Canonical: Q5PXQ6 (Residues: 1-293; Coverage: 100%)
Gene name: chqB
Sequence domains:
Structure domains: Aromatic compound dioxygenase

Ligands and Environments

6 bound ligands:
Ligand FE 2 x FE
Ligand CU 1 x CU
Ligand CL 1 x CL
Ligand SO4 2 x SO4
Ligand HGX 2 x HGX
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11, ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 46.283Å b: 84.978Å c: 83.923Å
α: 90° β: 92.84° γ: 90°
R-values:
R R work R free
0.195 0.193 0.247

Quick links

Citations

1 review citation

The ins and outs of ring-cleaving dioxygenases.
Vaillancourt et al. (2006)
22 other articles

3 mentions without citation

Hydroquinone: environmental pollution, toxicity, and microbial answers.
Enguita et al. (2013)
2 more