› 1tj3

X-ray diffraction
2.8Å resolution

X-Ray structure of the Sucrose-Phosphatase (SPP) from Synechocystis sp. PCC6803 in a closed conformation

Released:
DOI: 10.2210/pdb1tj3/pdb
PDB entry 1tj3 coloured by chain, front view.
PDB entry 1tj3 coloured by chain, side view.
PDB entry 1tj3 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
The structure of a cyanobacterial sucrose-phosphatase reveals the sugar tongs that release free sucrose in the cell.
Fieulaine S, Lunn JE, Borel F, Ferrer JL
Plant Cell 17 2049-58 (2005)
PMID: 15937230

Function and Biology Details

Reaction catalysed: 
Sucrose 6(F)-phosphate + H(2)O = sucrose + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
sucrose-phosphate phosphatase Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.79 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P74325 (Residues: 1-244; Coverage: 100%)
Gene name: slr0953
Sequence domains: Sucrose-6F-phosphate phosphohydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 68.75Å b: 68.75Å c: 268.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.176 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Trehalose metabolism in plants.
Lunn et al. (2014)
4 more

2 mentions without citation

Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications.
Rao et al. (2006)
1 more