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X-ray diffraction
2.7Å resolution

Crystal Structures of the ADP and ATP bound forms of the Bacillus Anti-sigma factor SpoIIAB in complex with the Anti-anti-sigma SpoIIAA:Poised for phosphorylation complex with ATP, crystal form II

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-sigma F factor Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 146 amino acids
Theoretical weight: 16.21 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32727 (Residues: 1-146; Coverage: 100%)
Gene name: spoIIAB
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain
Anti-sigma F factor antagonist Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 119 amino acids
Theoretical weight: 13.08 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32726 (Residues: 1-116; Coverage: 100%)
Gene name: spoIIAA
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4122
Unit cell:
a: 115.133Å b: 115.133Å c: 256.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.265
Expression system: Escherichia coli BL21(DE3)