1tid

X-ray diffraction
2.5Å resolution

Crystal Structures of the ADP and ATP bound forms of the Bacillus Anti-sigma factor SpoIIAB in complex with the Anti-anti-sigma SpoIIAA: Poised for phosphorylation complex with ATP, crystal form I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-sigma F factor Chains: A, C
Molecule details ›
Chains: A, C
Length: 136 amino acids
Theoretical weight: 15.41 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32727 (Residues: 1-136; Coverage: 93%)
Gene name: spoIIAB
Structure domains: Histidine kinase-like ATPase, C-terminal domain
Anti-sigma F factor antagonist Chains: B, D
Molecule details ›
Chains: B, D
Length: 119 amino acids
Theoretical weight: 13.27 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32726 (Residues: 1-116; Coverage: 100%)
Gene name: spoIIAA
Sequence domains: STAS domain
Structure domains: STAS domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41
Unit cell:
a: 49.465Å b: 49.465Å c: 265.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.266
Expression system: Escherichia coli BL21(DE3)