› 1tfr

X-ray diffraction
2.06Å resolution

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.61 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13319 (Residues: 1-305; Coverage: 100%)
Gene names: 33.2, das, rnh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 74.47Å b: 87.58Å c: 54.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.29
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Flap endonuclease 1: a central component of DNA metabolism.
Liu et al. (2004)
12 more

13 mentions without citation

Bacteriophage T4 genome.
Miller et al. (2003)
12 more