1tfo

X-ray diffraction
2.3Å resolution

Ribonuclease from Escherichia coli complexed with its inhibitor protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-D Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 11.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17998 (Residues: 595-697; Coverage: 15%)
Gene name: cda
Sequence domains: Colicin D
Structure domains: Nuclear Transport Factor 2; Chain: A,
Colicin-D immunity protein Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P11899 (Residues: 2-87; Coverage: 99%)
Gene name: cdi
Structure domains: Colicin D

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 56.171Å b: 56.171Å c: 149.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.258 0.309
Expression system: Escherichia coli