1tau

X-ray diffraction
3Å resolution

TAQ POLYMERASE (E.C.2.7.7.7)/DNA/B-OCTYLGLUCOSIDE COMPLEX

Released:
Source organism: Thermus aquaticus
Primary publication:
Structure of Taq polymerase with DNA at the polymerase active site.
Nature 382 278-81 (1996)
PMID: 8717047

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 832 amino acids
Theoretical weight: 94.05 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 1-832; Coverage: 100%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*TP*CP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
DNA (5'-D(*CP*GP*GP*AP*TP*CP*GP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 107.6Å b: 107.6Å c: 170.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.244 0.305
Expression systems:
  • Escherichia coli
  • Not provided