1t96

X-ray diffraction
1.85Å resolution

r106g kdo8ps with pep

Released:
Source organism: Aquifex aeolicus
Entry author: Gatti DL

Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.67 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66496 (Residues: 1-267; Coverage: 100%)
Gene names: aq_085, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 85.028Å b: 85.028Å c: 161.151Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.246
Expression system: Escherichia coli BL21(DE3)