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› 1t94

X-ray diffraction
2.4Å resolution

Crystal structure of the catalytic core of human DNA polymerase kappa

Released:
DOI: 10.2210/pdb1t94/pdb
PDB entry 1t94 coloured by chain, front view.
PDB entry 1t94 coloured by chain, side view.
PDB entry 1t94 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic core of human DNA polymerase kappa.
Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK
Structure 12 1395-404 (2004)
PMID: 15296733

Function and Biology Details

Reaction catalysed: 
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) +diphosphate.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase kappa Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 52.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBT6 (Residues: 68-526; Coverage: 53%)
Gene names: DINB1, POLK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B, APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 85.209Å b: 109.46Å c: 111.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.282
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.
Prakash et al. (2005)
12 more

9 mentions without citation

Translesion and Repair DNA Polymerases: Diverse Structure and Mechanism.
Yang et al. (2018)
8 more