› 1t94

X-ray diffraction
2.4Å resolution

Crystal structure of the catalytic core of human DNA polymerase kappa

Released:
DOI: 10.2210/pdb1t94/pdb
PDB 1t94 coloured by chain and viewed from the front.
PDB 1t94 coloured by chain and viewed from the side.
PDB 1t94 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic core of human DNA polymerase kappa.
Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK
Structure 12 1395-404 (2004)
PMID: 15296733

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase kappa Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 52.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBT6 (Residues: 68-526; Coverage: 53%)
Gene names: DINB1, POLK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B, APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 85.209Å b: 109.46Å c: 111.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.282
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Structural and Molecular Kinetic Features of Activities of DNA Polymerases.
Kuznetsova et al. (2022)
12 more

9 mentions without citation

Structure and function relationships in mammalian DNA polymerases.
Hoitsma et al. (2020)
8 more