Solution NMR

Solution structure of double-stranded RNA binding domain of S. cerevisiae RNase III (Rnt1p) in complex with the 5' terminal RNA hairpin of snR47 precursor


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chain: B
Molecule details ›
Chain: B
Length: 90 amino acids
Theoretical weight: 9.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
  • Canonical: Q02555 (Residues: 366-453; Coverage: 19%)
Gene names: RNT1, YM9408.01C, YM9959.21, YMR239C
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
5' terminal hairpin of snR47 precursor Chain: A
Molecule details ›
Chain: A
Length: 32 nucleotides
Theoretical weight: 10.31 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21
  • Not provided