1t39

X-ray diffraction
3.3Å resolution

HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE COVALENTLY CROSSLINKED TO DNA

Released:
Source organism: Homo sapiens
Primary publication:
DNA binding and nucleotide flipping by the human DNA repair protein AGT.
Nat. Struct. Mol. Biol. 11 714-20 (2004)
PMID: 15221026

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Methylated-DNA--protein-cysteine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 20.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16455 (Residues: 1-176; Coverage: 85%)
Gene name: MGMT
Sequence domains:
Structure domains:
5'-D(*GP*CP*CP*AP*TP*GP*(E1X)P*CP*TP*AP*GP*TP*A)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*TP*AP*CP*TP*AP*GP*CP*CP*AP*TP*GP*GP*C)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 228.145Å b: 41.25Å c: 70.629Å
α: 90° β: 103.14° γ: 90°
R-values:
R R work R free
0.301 0.296 0.344
Expression systems:
  • Escherichia coli
  • Not provided