› 1t27

X-ray diffraction
2.2Å resolution

THE STRUCTURE OF PITP COMPLEXED TO PHOSPHATIDYLCHOLINE

Released:
DOI: 10.2210/pdb1t27/pdb
PDB 1t27 coloured by chain and viewed from the front.
PDB 1t27 coloured by chain and viewed from the side.
PDB 1t27 coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine.
Yoder MD, Thomas LM, Tremblay JM, Oliver RL, Yarbrough LR, Helmkamp GM
J Biol Chem 276 9246-52 (2001)
PMID: 11104777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol transfer protein alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16446 (Residues: 1-271; Coverage: 100%)
Gene names: Pitpn, Pitpna
Sequence domains: Phosphatidylinositol transfer protein
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand PCW 1 x PCW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 43.914Å b: 73.773Å c: 48.185Å
α: 90° β: 114.73° γ: 90°
R-values:
R R work R free
0.205 0.205 0.25
Expression system: Escherichia coli

Quick links

Citations

27 review citations

GOLPH3: a Golgi phosphatidylinositol(4)phosphate effector that directs vesicle trafficking and drives cancer.
Kuna et al. (2019)
26 more

10 mentions without citation

The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands.
Radauer et al. (2008)
9 more