› 1t1u

X-ray diffraction
1.55Å resolution

Structural Insights and Functional Implications of Choline Acetyltransferase

Released:
DOI: 10.2210/pdb1t1u/pdb
PDB entry 1t1u coloured by chain, front view.
PDB entry 1t1u coloured by chain, side view.
PDB entry 1t1u coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural insights and functional implications of choline acetyltransferase.
Govindasamy L, Pedersen B, Lian W, Kukar T, Gu Y, Jin S, Agbandje-McKenna M, Wu D, McKenna R
J Struct Biol 148 226-35 (2004)
PMID: 15477102

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + choline = CoA + O-acetylcholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline O-acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 71.91 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32738 (Residues: 2-640; Coverage: 100%)
Gene name: Chat
Sequence domains: Choline/Carnitine o-acyltransferase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 138.949Å b: 77.505Å c: 59.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 0.227
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural insight into function and regulation of carnitine palmitoyltransferase.
Rufer et al. (2009)
1 more

2 mentions without citation

Carnitine palmitoyl transferase-1A (CPT1A): a new tumor specific target in human breast cancer.
Pucci et al. (2016)
1 more