1t1h

Solution NMR

NMR solution structure of the U box domain from AtPUB14, an armadillo repeat containing protein from Arabidopsis thaliana

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biological process:
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U-box domain-containing protein 14 Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.76 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VZ40 (Residues: 249-321; Coverage: 12%)
Gene names: At3g54850, F28P10.170, PUB14
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Chemical shifts: BMR6265  
Expression system: Escherichia coli BL21(DE3)