› 1t19

X-ray diffraction
1.6Å resolution

Early intermediate IE2 from time-resolved crystallography of the E46Q mutant of PYP

Released:
DOI: 10.2210/pdb1t19/pdb
PDB 1t19 coloured by chain and viewed from the front.
PDB 1t19 coloured by chain and viewed from the side.
PDB 1t19 coloured by chain and viewed from the top.
Source organism: Halorhodospira halophila
Primary publication:
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein.
Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K
Structure 13 55-63 (2005)
PMID: 15642261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P63
Unit cell:
a: 66.917Å b: 66.917Å c: 41.002Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.219
Expression system: Escherichia coli

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Citations

6 review citations

A short history of structure based research on the photocycle of photoactive yellow protein.
Schmidt M. (2017)
5 more