X-ray diffraction
6.21Å resolution

Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 51.47 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
T-RNA (76-MER) Chains: E, F
Molecule details ›
Chains: E, F
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P321
Unit cell:
a: 218.403Å b: 218.403Å c: 170.263Å
α: 90° β: 90° γ: 120°
R R work R free
0.361 0.36 0.38
Expression systems:
  • Escherichia coli BL21
  • Not provided