› 1syk

X-ray diffraction
2.8Å resolution

Crystal structure of E230Q mutant of cAMP-dependent protein kinase reveals unexpected apoenzyme conformation

Released:
DOI: 10.2210/pdb1syk/pdb
PDB entry 1syk coloured by chain, front view.
PDB entry 1syk coloured by chain, side view.
PDB entry 1syk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the E230Q mutant of cAMP-dependent protein kinase reveals an unexpected apoenzyme conformation and an extended N-terminal A helix.
Wu J, Yang J, Kannan N, Xuong NH, Ten Eyck LF, Taylor SS
Protein Sci 14 2871-9 (2005)
PMID: 16253959

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P42
Unit cell:
a: 120.3Å b: 120.3Å c: 58.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.249
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Networks for the allosteric control of protein kinases.
Shi et al. (2006)
1 more

9 mentions without citation

A conserved non-canonical motif in the pseudoactive site of the ROP5 pseudokinase domain mediates its effect on Toxoplasma virulence.
Reese et al. (2011)
8 more