X-ray diffraction
2.6Å resolution

Crystal Structural Analysis of Active Site Mutant Q189E of LgtC


Function and Biology Details

Reaction catalysed:
UDP-alpha-D-galactose + [lipopolysaccharide] = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide]
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
LgtC Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 35.79 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21
  • Canonical: Q93EK7 (Residues: 1-311; Coverage: null%)
Gene name: lgtC
Sequence domains: Glycosyl transferase family 8
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 39.85Å b: 75.934Å c: 86.905Å
α: 90° β: 90° γ: 90°
R R work R free
0.217 0.217 0.283
Expression system: Escherichia coli BL21