X-ray diffraction
2.3Å resolution

Crystal Structure of the DegS stress sensor

Source organism: Escherichia coli
Entry authors: Wilken C, Kitzing K, Kurzbauer R, Ehrmann M, Clausen T

Function and Biology Details

Reaction catalysed:
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Serine endoprotease DegS Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 320 amino acids
Theoretical weight: 34.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P0AEE3 (Residues: 43-355; Coverage: 88%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 207.681Å b: 143.062Å c: 41.529Å
α: 90° β: 90.07° γ: 90°
R R work R free
0.201 0.198 0.248
Expression system: Escherichia coli BL21(DE3)