PDBe 1soj

X-ray diffraction
2.9Å resolution

CATALYTIC DOMAIN OF HUMAN PHOSPHODIESTERASE 3B IN COMPLEX WITH IBMX

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 420 amino acids
Theoretical weight: 48.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13370 (Residues: 654-1073; Coverage: 38%)
Gene name: PDE3B
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: C2
Unit cell:
a: 275.046Å b: 147.078Å c: 253.485Å
α: 90° β: 109.84° γ: 90°
R-values:
R R work R free
0.235 0.231 0.249
Expression system: Escherichia coli