› 1slf

X-ray diffraction
1.76Å resolution

APOSTREPTAVIDIN, PH 5.6, TWO MOLECULES OF (SO4)2 BOUND AT THE BIOTIN BINDING SITE

Released:
DOI: 10.2210/pdb1slf/pdb
PDB entry 1slf coloured by chain, front view.
PDB entry 1slf coloured by chain, side view.
PDB entry 1slf coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Binding to protein targets of peptidic leads discovered by phage display: crystal structures of streptavidin-bound linear and cyclic peptide ligands containing the HPQ sequence.
Katz BA
Biochemistry 34 15421-9 (1995)
PMID: 7492542

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: B, D
Molecule details ›
Chains: B, D
Length: 135 amino acids
Theoretical weight: 14.18 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 25-159; Coverage: 85%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 95.43Å b: 106.12Å c: 47.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.202

Quick links

Citations

11 review citations

Selection-based discovery of druglike macrocyclic peptides.
Passioura et al. (2014)
10 more

3 mentions without citation

Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1.
Xu et al. (2018)
2 more