1sky

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP synthase subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 502 amino acids
Theoretical weight: 54.65 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P09219 (Residues: 1-502; Coverage: 100%)
Gene name: atpA
Sequence domains:
Structure domains:
ATP synthase subunit beta Chain: E
Molecule details ›
Chain: E
Length: 473 amino acids
Theoretical weight: 52 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P07677 (Residues: 1-473; Coverage: 100%)
Gene name: atpD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P213
Unit cell:
a: 159.5Å b: 159.5Å c: 159.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.299
Expression system: Escherichia coli