Assemblies
Assembly Name:
DNA-directed DNA polymerase and Thioredoxin 1 and DNA
Multimeric state:
hetero tetramer
Accessible surface area:
36103.84 Å2
Buried surface area:
8318.41 Å2
Dissociation area:
999.43
Å2
Dissociation energy (ΔGdiss):
6.79
kcal/mol
Dissociation entropy (TΔSdiss):
11.91
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-114599
Macromolecules
Chain: A
Length: 698 amino acids
Theoretical weight: 79.09 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
Pfam: DNA polymerase family A
InterPro:
Length: 698 amino acids
Theoretical weight: 79.09 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
- Canonical: P00581 (Residues: 1-704; Coverage: 99%)
Pfam: DNA polymerase family A
InterPro:
- Ribonuclease H-like superfamily
- DNA-directed DNA polymerase T7
- Ribonuclease H superfamily
- YprB, ribonuclease H-like domain
- DNA polymerase A
- DNA/RNA polymerase superfamily
- DNA-directed DNA polymerase, family A, palm domain
- DNA-directed DNA polymerase, family A, conserved site
- Ribonuclease H-like superfamily/Ribonuclease H
- Taq DNA Polymerase; Chain T, domain 4
- Alpha-Beta Plaits
- 5' to 3' exonuclease, C-terminal subdomain
Chain: B
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Pfam: Thioredoxin
InterPro:
CATH: Glutaredoxin
SCOP: Thioltransferase
Length: 108 amino acids
Theoretical weight: 11.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Pfam: Thioredoxin
InterPro:
CATH: Glutaredoxin
SCOP: Thioltransferase
Name:
5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*C*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(2DA))-3'
Representative chains: P
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Representative chains: P
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Name:
5'-D(*CP*CP*CP*TP*TP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3'
Representative chains: T
Length: 26 nucleotides
Theoretical weight: 7.91 KDa
Representative chains: T
Length: 26 nucleotides
Theoretical weight: 7.91 KDa