1ska

X-ray diffraction
1.69Å resolution

Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics

Released:

Function and Biology Details

Reaction catalysed:
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phytase A Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 48.32 KDa
Source organism: Aspergillus fumigatus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O00092 (Residues: 27-465; Coverage: 100%)
Gene names: AFUA_4G08630, phyA, phyA3
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 39.938Å b: 95.225Å c: 98.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.198
Expression system: Komagataella pastoris