1shw

X-ray diffraction
2.2Å resolution

EphB2 / EphrinA5 Complex Structure

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Ephrin-A5 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 16.32 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: O08543 (Residues: 28-165; Coverage: 66%)
Gene names: Efna5, Epl7, Eplg7, Lerk7
Structure domains: Cupredoxins - blue copper proteins
Ephrin type-B receptor 2 Chain: B
Molecule details ›
Chain: B
Length: 181 amino acids
Theoretical weight: 20.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54763 (Residues: 19-199; Coverage: 19%)
Gene names: Ephb2, Epth3, Nuk, Sek3
Sequence domains: Ephrin receptor ligand binding domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 37.89Å b: 88.97Å c: 122.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.258 0.305
Expression systems:
  • Homo sapiens
  • Escherichia coli