1shp › Citations

The NMR solution structure of a Kunitz-type proteinase inhibitor from the sea anemone Stichodactyla helianthus.

Antuch W, Berndt KD, Chávez MA, Delfín J, Wüthrich K
Eur J Biochem 212 675-84 (1993)
Cited: 51 times
EuropePMC logo PMID: 8462542

Abstract

The solution structure of a 55-amino-acid Kunitz-type proteinase inhibitor, ShPI, purified from the Caribbean sea anemone Stichodactyla helianthus, was determined by NMR spectroscopy. Nearly complete sequence-specific 1H-NMR assignments were obtained at pH 4.6 and 36 degrees C, and stereo-specific assignments were determined for 23 pairs of diastereotopic substituents. A data set of 666 upper distance limit constraints and 122 dihedral angle constraints collected on this basis was used as input for a structure calculation with the program DIANA. Following energy minimization with the program OPAL, the average root-mean-square diviation (RMSD) of the 20 DIANA conformers used to represent the solution structure relative to the mean structure is 61 pm for all backbone atoms N, C alpha and C', and 106 pm for all heavy atoms of residues 2-53. This high-quality solution structure of ShPI has a nearly identical molecular architecture as the bovine pancreatic trypsin inhibitor (BPTI), despite a mere 35% of sequence similarity between the two proteins. Exchange rates measured for 48 out of the 51 backbone amide protons showed that the positions of 20 slowly exchanging amide protons correlate well with hydrogen bonds involving these protons in the energy-minimized solution structure. The solution structure of ShPI is compared to the four homologous proteins for which the three-dimensional structure is also available.

Reviews - 1shp mentioned but not cited (2)

  1. Sea anemone (Cnidaria, Anthozoa, Actiniaria) toxins: an overview. Frazão B, Vasconcelos V, Antunes A. Mar Drugs 10 1812-1851 (2012)
  2. Protease inhibitors from marine venomous animals and their counterparts in terrestrial venomous animals. Mourão CB, Schwartz EF. Mar Drugs 11 2069-2112 (2013)

Articles - 1shp mentioned but not cited (12)

  1. Analgesic compound from sea anemone Heteractis crispa is the first polypeptide inhibitor of vanilloid receptor 1 (TRPV1). Andreev YA, Kozlov SA, Koshelev SG, Ivanova EA, Monastyrnaya MM, Kozlovskaya EP, Grishin EV. J Biol Chem 283 23914-23921 (2008)
  2. Evolution, expansion and expression of the Kunitz/BPTI gene family associated with long-term blood feeding in Ixodes Scapularis. Dai SX, Zhang AD, Huang JF. BMC Evol Biol 12 4 (2012)
  3. TRPV1 activation power can switch an action mode for its polypeptide ligands. Nikolaev MV, Dorofeeva NA, Komarova MS, Korolkova YV, Andreev YA, Mosharova IV, Grishin EV, Tikhonov DB, Kozlov SA. PLoS One 12 e0177077 (2017)
  4. Kunitz-Type Peptide HCRG21 from the Sea Anemone Heteractis crispa Is a Full Antagonist of the TRPV1 Receptor. Monastyrnaya M, Peigneur S, Zelepuga E, Sintsova O, Gladkikh I, Leychenko E, Isaeva M, Tytgat J, Kozlovskaya E. Mar Drugs 14 (2016)
  5. Atypical reactive center Kunitz-type inhibitor from the sea anemone Heteractis crispa. Gladkikh I, Monastyrnaya M, Leychenko E, Zelepuga E, Chausova V, Isaeva M, Anastyuk S, Andreev Y, Peigneur S, Tytgat J, Kozlovkaya E. Mar Drugs 10 1545-1565 (2012)
  6. New Kunitz-Type HCRG Polypeptides from the Sea Anemone Heteractis crispa. Gladkikh I, Monastyrnaya M, Zelepuga E, Sintsova O, Tabakmakher V, Gnedenko O, Ivanov A, Hua KF, Kozlovskaya E. Mar Drugs 13 6038-6063 (2015)
  7. A new multigene HCIQ subfamily from the sea anemone Heteractis crispa encodes Kunitz-peptides exhibiting neuroprotective activity against 6-hydroxydopamine. Kvetkina A, Leychenko E, Chausova V, Zelepuga E, Chernysheva N, Guzev K, Pislyagin E, Yurchenko E, Menchinskaya E, Aminin D, Kaluzhskiy L, Ivanov A, Peigneur S, Tytgat J, Kozlovskaya E, Isaeva M. Sci Rep 10 4205 (2020)
  8. Kunitz-Type Peptides from the Sea Anemone Heteractis crispa Demonstrate Potassium Channel Blocking and Anti-Inflammatory Activities. Gladkikh I, Peigneur S, Sintsova O, Lopes Pinheiro-Junior E, Klimovich A, Menshov A, Kalinovsky A, Isaeva M, Monastyrnaya M, Kozlovskaya E, Tytgat J, Leychenko E. Biomedicines 8 E473 (2020)
  9. A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity. Loret EP, Luis J, Nuccio C, Villard C, Mansuelle P, Lebrun R, Villard PH. Mar Drugs 16 (2018)
  10. Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus. García-Fernández R, Pons T, Meyer A, Perbandt M, González-González Y, Gil D, de los Angeles Chávez M, Betzel C, Redecke L. Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1289-1293 (2012)
  11. Design, synthesis, biological evaluation and molecular dynamics studies of 4-thiazolinone derivatives as protein tyrosine phosphatase 1B (PTP1B) inhibitors. Liu WS, Wang RR, Yue H, Zheng ZH, Lu XH, Wang SQ, Dong WL, Wang RL. J Biomol Struct Dyn 38 3814-3824 (2020)
  12. Polar Desolvation and Position 226 of Pancreatic and Neutrophil Elastases Are Crucial to their Affinity for the Kunitz-Type Inhibitors ShPI-1 and ShPI-1/K13L. Hernández González JE, García-Fernández R, Valiente PA. PLoS One 10 e0137787 (2015)


Reviews citing this publication (8)

  1. The toxicogenomic multiverse: convergent recruitment of proteins into animal venoms. Fry BG, Roelants K, Champagne DE, Scheib H, Tyndall JD, King GF, Nevalainen TJ, Norman JA, Lewis RJ, Norton RS, Renjifo C, de la Vega RC. Annu Rev Genomics Hum Genet 10 483-511 (2009)
  2. Structure and distribution of modules in extracellular proteins. Bork P, Downing AK, Kieffer B, Campbell ID. Q Rev Biophys 29 119-167 (1996)
  3. Molecular diversity of spider venom. Vassilevski AA, Kozlov SA, Grishin EV. Biochemistry (Mosc) 74 1505-1534 (2009)
  4. Peptide toxins in sea anemones: structural and functional aspects. Honma T, Shiomi K. Mar Biotechnol (NY) 8 1-10 (2006)
  5. Use of venom peptides to probe ion channel structure and function. Dutertre S, Lewis RJ. J Biol Chem 285 13315-13320 (2010)
  6. Structures of sea anemone toxins. Norton RS. Toxicon 54 1075-1088 (2009)
  7. Sea Anemone Toxins: A Structural Overview. Madio B, King GF, Undheim EAB. Mar Drugs 17 (2019)
  8. The Anemonia viridis Venom: Coupling Biochemical Purification and RNA-Seq for Translational Research. Nicosia A, Mikov A, Cammarata M, Colombo P, Andreev Y, Kozlov S, Cuttitta A. Mar Drugs 16 (2018)

Articles citing this publication (29)

  1. The ornithodorin-thrombin crystal structure, a key to the TAP enigma? van de Locht A, Stubbs MT, Bode W, Friedrich T, Bollschweiler C, Höffken W, Huber R. EMBO J 15 6011-6017 (1996)
  2. Isolation and characterization of novel inducible serine protease inhibitors from larval hemolymph of the greater wax moth Galleria mellonella. Fröbius AC, Kanost MR, Götz P, Vilcinskas A. Eur J Biochem 267 2046-2053 (2000)
  3. Purification, characterization and immobilization of proteinase inhibitors from Stichodactyla helianthus. Delfín J, Martínez I, Antuch W, Morera V, González Y, Rodríguez R, Márquez M, Saroyán A, Larionova N, Díaz J, Padrón G, Chávez M. Toxicon 34 1367-1376 (1996)
  4. Novel peptide toxins from the sea anemone Stichodactyla haddoni. Honma T, Kawahata S, Ishida M, Nagai H, Nagashima Y, Shiomi K. Peptides 29 536-544 (2008)
  5. Evolutionary trace analysis of the Kunitz/BPTI family of proteins: functional divergence may have been based on conformational adjustment. Pritchard L, Dufton MJ. J Mol Biol 285 1589-1607 (1999)
  6. Development of a rational nomenclature for naming peptide and protein toxins from sea anemones. Oliveira JS, Fuentes-Silva D, King GF. Toxicon 60 539-550 (2012)
  7. Polypeptide modulators of TRPV1 produce analgesia without hyperthermia. Andreev YA, Kozlov SA, Korolkova YV, Dyachenko IA, Bondarenko DA, Skobtsov DI, Murashev AN, Kotova PD, Rogachevskaja OA, Kabanova NV, Kolesnikov SS, Grishin EV. Mar Drugs 11 5100-5115 (2013)
  8. NMR solution structure of the recombinant tick anticoagulant protein (rTAP), a factor Xa inhibitor from the tick Ornithodoros moubata. Antuch W, Güntert P, Billeter M, Hawthorne T, Grossenbacher H, Wüthrich K. FEBS Lett 352 251-257 (1994)
  9. Primary structure of a potassium channel toxin from the sea anemone Actinia equina. Minagawa S, Ishida M, Nagashima Y, Shiomi K. FEBS Lett 427 149-151 (1998)
  10. A new toxin from the sea anemone Condylactis gigantea with effect on sodium channel inactivation. Ständker L, Béress L, Garateix A, Christ T, Ravens U, Salceda E, Soto E, John H, Forssmann WG, Aneiros A. Toxicon 48 211-220 (2006)
  11. [New polypeptide components from the Heteractis crispa sea anemone with analgesic activity]. Kozlov SA, Andreev IaA, Murashev AN, Skobtsov DI, D'iachenko IA, Grishin EV. Bioorg Khim 35 789-798 (2009)
  12. A new multigene superfamily of Kunitz-type protease inhibitors from sea anemone Heteractis crispa. Isaeva MP, Chausova VE, Zelepuga EA, Guzev KV, Tabakmakher VM, Monastyrnaya MM, Kozlovskaya EP. Peptides 34 88-97 (2012)
  13. Conformational and functional variability supported by the BPTI fold: solution structure of the Ca2+ channel blocker calcicludine. Gilquin B, Lecoq A, Desné F, Guenneugues M, Zinn-Justin S, Ménez A. Proteins 34 520-532 (1999)
  14. Effects of serine protease inhibitors on viability and morphology of Leishmania (Leishmania) amazonensis promastigotes. Silva-Lopez RE, Morgado-Díaz JA, Chávez MA, Giovanni-De-Simone S. Parasitol Res 101 1627-1635 (2007)
  15. Kunitz-type protease inhibitors from acrorhagi of three species of sea anemones. Minagawa S, Sugiyama M, Ishida M, Nagashima Y, Shiomi K. Comp Biochem Physiol B Biochem Mol Biol 150 240-245 (2008)
  16. NMR structure determination of tick anticoagulant peptide (TAP). Lim-Wilby MS, Hallenga K, de Maeyer M, Lasters I, Vlasuk GP, Brunck TK. Protein Sci 4 178-186 (1995)
  17. Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein. Nagy A, Trexler M, Patthy L. Eur J Biochem 270 2101-2107 (2003)
  18. An unexpected inhibitory activity of Kunitz-type serine proteinase inhibitor derived from Boophilus microplus trypsin inhibitor on cathepsin L. Sasaki SD, Cotrin SS, Carmona AK, Tanaka AS. Biochem Biophys Res Commun 341 266-272 (2006)
  19. Effects of a marine serine protease inhibitor on viability and morphology of Trypanosoma cruzi, the agent of Chagas disease. de Almeida Nogueira NP, Morgado-Díaz JA, Menna-Barreto RF, Paes MC, da Silva-López RE. Acta Trop 128 27-35 (2013)
  20. Three-dimensional Structure of a Kunitz-type Inhibitor in Complex with an Elastase-like Enzyme. García-Fernández R, Perbandt M, Rehders D, Ziegelmüller P, Piganeau N, Hahn U, Betzel C, Chávez Mde L, Redecke L. J Biol Chem 290 14154-14165 (2015)
  21. Recombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris. Gil DF, García-Fernández R, Alonso-del-Rivero M, Lamazares E, Pérez M, Varas L, Díaz J, Chávez MA, González-González Y, Mansur M. FEMS Yeast Res 11 575-586 (2011)
  22. [Interaction of sea amemone Heteractis crispa Kunitz type polypeptides with pain vanilloid receptor TRPV1: in silico investigation]. Zelepuga EA, Tabakmakher VM, Chausova VE, monastyrnaia MM, Isaeva MP, Kozlovskaia ÉP. Bioorg Khim 38 185-198 (2012)
  23. Elucidation of the origin of multiple conformations of the human alpha 3-chain type VI collagen C-terminal Kunitz domain: the reorientation of the Trp21 ring. Sørensen MD, Kristensen SM, Bjørn S, Norris K, Olsen O, Led JJ. J Biomol NMR 8 391-403 (1996)
  24. Expression and purification of natural N-terminal recombinant bovine pancreatic trypsin inhibitor from Pichia pastoris. Yang L, Dong W, He J, Ren X, Yan W. Biol Pharm Bull 31 1680-1685 (2008)
  25. Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis. Kong HJ, Lee YJ, Park IS, Lee WW, Kim YO, Nam BH, Kim WJ, Jung H, Jeon YJ, An CM, Lee SJ. Fish Shellfish Immunol 35 1025-1029 (2013)
  26. Recombinant bovine pancreatic trypsin inhibitor protects the liver from carbon tetrachloride-induced acute injury in mice. Yang L, Dong W, Yan F, Ren X, Hao X. J Pharm Pharmacol 62 332-338 (2010)
  27. Secretion and assembly of calicivirus-like particles in high-cell-density yeast fermentations: strategies based on a recombinant non-specific BPTI-Kunitz-type protease inhibitor. Fernández E, Toledo JR, Mansur M, Sánchez O, Gil DF, González-González Y, Lamazares E, Fernández Y, Parra F, Farnós O. Appl Microbiol Biotechnol 99 3875-3886 (2015)
  28. Peptides from the Sea Anemone Metridium senile with Modified Inhibitor Cystine Knot (ICK) Fold Inhibit Nicotinic Acetylcholine Receptors. Kasheverov IE, Logashina YA, Kornilov FD, Lushpa VA, Maleeva EE, Korolkova YV, Yu J, Zhu X, Zhangsun D, Luo S, Stensvåg K, Kudryavtsev DS, Mineev KS, Andreev YA. Toxins (Basel) 15 28 (2022)
  29. Recombinant bovine pancreatic trypsin inhibitor protects the liver from carbon tetrachloride-induced chronic injury in rats. Dong W, Lv B, Wei F, Yang L. Pharm Biol 51 1298-1303 (2013)


Related citations provided by authors (1)

  1. Amino Acid Sequence and Three-Dimensional Model of a Serine Proteinase Inhibitor from Stichodactyla Helianthus. Antuch W, Dominguez R, Rodriguez R, Delfin J, Morera V, Diaz J, Chavez M, Dideberg O, Padron G To be published -

Quick links