X-ray diffraction
2.95Å resolution

Crystal Structure of Norwalk Virus Polymerase (MgSO4 crystal form)


Function and Biology Details

Reactions catalysed:
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
NTPase Chains: A, B
Molecule details ›
Chains: A, B
Length: 510 amino acids
Theoretical weight: 56.81 KDa
Source organism: Norwalk virus
Expression system: Escherichia coli
  • Canonical: Q70ET3 (Residues: 329-838; Coverage: 61%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 105.05Å b: 109.14Å c: 112.044Å
α: 90° β: 90° γ: 90°
R R work R free
0.219 0.215 0.28
Expression system: Escherichia coli