1sfc

X-ray diffraction
2.4Å resolution

NEURONAL SYNAPTIC FUSION COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 2 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 96 amino acids
Theoretical weight: 10.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 1-96; Coverage: 83%)
Gene names: Syb2, Vamp2
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 83 amino acids
Theoretical weight: 9.6 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 180-262; Coverage: 29%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 83 amino acids
Theoretical weight: 9.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 1-83; Coverage: 40%)
  • Best match: P60881-2 (Residues: 1-83)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 87 amino acids
Theoretical weight: 9.86 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 120-206; Coverage: 42%)
Gene names: Snap, Snap25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: I222
Unit cell:
a: 98.715Å b: 111.071Å c: 198.844Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.265 0.303
Expression system: Escherichia coli