› 1sek

X-ray diffraction
2.1Å resolution

THE STRUCTURE OF ACTIVE SERPIN K FROM MANDUCA SEXTA AND A MODEL FOR SERPIN-PROTEASE COMPLEX FORMATION

Released:
DOI: 10.2210/pdb1sek/pdb
PDB entry 1sek coloured by chain, front view.
PDB entry 1sek coloured by chain, side view.
PDB entry 1sek coloured by chain, top view.
Source organism: Manduca sexta
Primary publication:
The structure of active serpin 1K from Manduca sexta.
Li J, Wang Z, Canagarajah B, Jiang H, Kanost M, Goldsmith EJ
Structure 7 103-9 (1999)
PMID: 10368276

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serpin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 42.27 KDa
Source organism: Manduca sexta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25526 (Residues: 15-392; Coverage: 100%)
Gene name: serpin 1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 125.96Å b: 42.05Å c: 76.05Å
α: 90° β: 117.6° γ: 90°
R-values:
R R work R free
0.199 0.199 0.26
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Innate immune responses of a lepidopteran insect, Manduca sexta.
Kanost et al. (2004)
7 more

8 mentions without citation

Serpins in arthropod biology.
Meekins et al. (2017)
7 more