1sd9

X-ray diffraction
1.65Å resolution

ARSENATE REDUCTASE C12S MUTANT +0.4M ARSENATE FROM E. COLI

Released:

Function and Biology Details

Reaction catalysed:
Arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arsenate reductase Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08692 (Residues: 1-141; Coverage: 100%)
Gene name: arsC
Sequence domains: ArsC family
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6122
Unit cell:
a: 86.59Å b: 86.59Å c: 115.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.214
Expression system: Escherichia coli BL21(DE3)