› 1s9k

X-ray diffraction
3.1Å resolution

Crystal Structure of Human NFAT1 and Fos-Jun on the IL-2 ARRE1 Site

Released:
DOI: 10.2210/pdb1s9k/pdb
PDB 1s9k coloured by chain and viewed from the front.
PDB 1s9k coloured by chain and viewed from the side.
PDB 1s9k coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Wang D, Stroud JC, Chen L

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chain: C
Molecule details ›
Chain: C
Length: 280 amino acids
Theoretical weight: 31.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13469 (Residues: 399-678; Coverage: 30%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Proto-oncogene c-Fos Chain: D
Molecule details ›
Chain: D
Length: 53 amino acids
Theoretical weight: 6.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01100 (Residues: 140-192; Coverage: 14%)
Gene names: FOS, G0S7
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Transcription factor AP-1 Chain: E
Molecule details ›
Chain: E
Length: 52 amino acids
Theoretical weight: 6.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05412 (Residues: 257-308; Coverage: 16%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Human IL-2 ARRE1 Promoter Element, Plus Strand Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Human IL-2 ARRE1 Promoter Element, Minus Strand Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.05 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 64.769Å b: 86.26Å c: 84.044Å
α: 90° β: 111.24° γ: 90°
R-values:
R R work R free
0.242 0.242 0.275
Expression system: Escherichia coli

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