1s97

X-ray diffraction
2.4Å resolution

DPO4 with GT mismatch

Released:
Source organism: Saccharolobus solfataricus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase IV Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 352 amino acids
Theoretical weight: 40.26 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 1-352; Coverage: 100%)
Gene names: SSO2448, dbh, dpo4
Sequence domains:
Structure domains:
5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*CP*TP*G)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 nucleotides
Theoretical weight: 4.12 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided
5'-D(*T*TP*CP*AP*GP*TP*AP*GP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3' Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 18 nucleotides
Theoretical weight: 5.38 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 104.433Å b: 100.644Å c: 110.426Å
α: 90° β: 94.89° γ: 90°
R-values:
R R work R free
0.236 0.218 0.269
Expression systems:
  • Escherichia coli
  • Not provided