› 1s8j

X-ray diffraction
2.3Å resolution

Nitrate-bound D85S mutant of bacteriorhodopsin

Released:
DOI: 10.2210/pdb1s8j/pdb
PDB entry 1s8j coloured by chain, front view.
PDB entry 1s8j coloured by chain, side view.
PDB entry 1s8j coloured by chain, top view.
Source organism: Halobacterium sp.
Primary publication:
Specificity of anion binding in the substrate pocket of bacteriorhodopsin.
Facciotti MT, Cheung VS, Lunde CS, Rouhani S, Baliga NS, Glaeser RM
Biochemistry 43 4934-43 (2004)
PMID: 15109251

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.9 KDa
Source organism: Halobacterium sp.
Expression system: Halobacterium salinarum NRC-1
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand NO3 1 x NO3
Ligand RET 1 x RET
Ligand LI1 7 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 52.95Å b: 121.08Å c: 74.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.235
Expression system: Halobacterium salinarum NRC-1

Quick links

Citations

2 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
1 more

3 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
2 more