› 1s89

X-ray diffraction
2.22Å resolution

H98N Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid

Released:
DOI: 10.2210/pdb1s89/pdb
PDB 1s89 coloured by chain and viewed from the front.
PDB 1s89 coloured by chain and viewed from the side.
PDB 1s89 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Mutagenic studies on histidine 98 of methylglyoxal synthase: effects on mechanism and conformational change.
Marks GT, Susler M, Harrison DH
Biochemistry 43 3802-13 (2004)
PMID: 15049687

Function and Biology Details

Reaction catalysed: 
Glycerone phosphate = methylglyoxal + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylglyoxal synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 152 amino acids
Theoretical weight: 16.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A731 (Residues: 1-152; Coverage: 100%)
Gene names: JW5129, b0963, mgsA, yccG
Sequence domains: MGS-like domain
Structure domains: Methylglyoxal synthase-like domain

Ligands and Environments

1 bound ligand:
Ligand PGA 6 x PGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 53.479Å b: 129.497Å c: 178.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.229
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Selective prediction of interaction sites in protein structures with THEMATICS.
Wei et al. (2007)
7 more

1 mention without citation

Automated protein motif generation in the structure-based protein function prediction tool ProMOL.
Osipovitch et al. (2015)