X-ray diffraction
2.22Å resolution

H98N Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid


Function and Biology Details

Reaction catalysed:
Glycerone phosphate = methylglyoxal + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Methylglyoxal synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 152 amino acids
Theoretical weight: 16.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P0A731 (Residues: 1-152; Coverage: 100%)
Gene names: JW5129, b0963, mgsA, yccG
Sequence domains: MGS-like domain
Structure domains: Methylglyoxal synthase-like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 53.479Å b: 129.497Å c: 178.22Å
α: 90° β: 90° γ: 90°
R R work R free
0.19 0.19 0.229
Expression system: Escherichia coli