1s46

X-ray diffraction
2.2Å resolution

Covalent intermediate of the E328Q amylosucrase mutant

Released:

Function and Biology Details

Reaction catalysed:
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.53 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 13-636; Coverage: 98%)
Gene name: ams
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21212
Unit cell:
a: 96.253Å b: 116.63Å c: 60.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.184 0.221
Expression system: Escherichia coli