› 1s21

X-ray diffraction
2Å resolution

Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae

Released:
DOI: 10.2210/pdb1s21/pdb
PDB entry 1s21 coloured by chain, front view.
PDB entry 1s21 coloured by chain, side view.
PDB entry 1s21 coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. phaseolicola
Primary publication:
Crystal structures of the type III effector protein AvrPphF and its chaperone reveal residues required for plant pathogenesis.
Singer AU, Desveaux D, Betts L, Chang JH, Nimchuk Z, Grant SR, Dangl JL, Sondek J
Structure 12 1669-81 (2004)
PMID: 15341731

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191747 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF2 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.05 KDa
Source organism: Pseudomonas savastanoi pv. phaseolicola
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K2L5 (Residues: 1-204; Coverage: 100%)
Sequence domains: AvrPphF-ORF-2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 38.689Å b: 68.334Å c: 88.365Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.241
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Effectors of Filamentous Plant Pathogens: Commonalities amid Diversity.
Franceschetti et al. (2017)
14 more

4 mentions without citation

The natural history of ADP-ribosyltransferases and the ADP-ribosylation system.
Aravind et al. (2015)
3 more