› 1rzf

X-ray diffraction
1.7Å resolution

Crystal structure of Human anti-HIV-1 GP120-reactive antibody E51

Released:
DOI: 10.2210/pdb1rzf/pdb
PDB 1rzf coloured by chain and viewed from the front.
PDB 1rzf coloured by chain and viewed from the side.
PDB 1rzf coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Huang CC, Venturi M, Majeed S, Moore MJ, Phogat S, Zhang MY, Dimitrov DS, Hendrickson WA, Robinson J, Sodroski J, Wyatt R, Choe H, Farzan M, Kwong PD
Proc. Natl. Acad. Sci. U.S.A. 101 2706-11 (2004)
PMID: 14981267

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab E51 light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.35 KDa
Source organism: Homo sapiens
Expression system: Human gammaherpesvirus 4
Structure domains: Immunoglobulins
Fab E51 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 235 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: Human gammaherpesvirus 4
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 13 x GOL
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 64.77Å b: 71.608Å c: 99.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.232
Expression system: Human gammaherpesvirus 4

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Citations

32 review citations

On the irrationality of rational design of an HIV vaccine in light of protein intrinsic disorder.
Uversky VN. (2021)
31 more

8 mentions without citation

Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Huang et al. (2004)
7 more